. 2016 Apr 13;6:24232. doi: 10.1038/srep24232

Table 2. ELISA screening of candidate peptides identified by NGPD as being enriched against IgY from chickens infected with the serovar S. Enteritidis.

Peptide sequence^a	In silico assessment^b	ELISA assessment
Peptide sequence^a	In silico assessment^b	AUC of ROC^c	P value^c
AEGEFRESPQNSLA	2/9	0.69	0.030
AEGEFLPRPIQPTP	2/9	0.60	0.260
AEGEFAPQGPAPTA	2/9	0.68	0.045
AEGEFPRNQEHFAM	2/9	0.60	0.260
AEGEFRSQSRRFHL	2/9	0.64	0.127
AEGEFEPQQSARPS	2/9	0.77	0.002
AEGEFAPHMQFKTS	2/9	0.67	0.050
AEGEFWARTHVQKV	2/9	0.67	0.059
CSTRRPIQTTCG	2/9	0.76	0.003
AEGEFFVNRALINQ	2/9	0.83	<0.001
AEGEFAPSRQHRSW	2/9	0.71	0.021
AEGEFQGASGRSCR	2/9	0.54	0.661
AEGEFMALLNQSYP	2/9	0.67	0.063
CQPLQSINITCG	2/9	0.79	0.001
AEGEFQALPKREPP	2/9	0.69	0.034
Control peptide		0.54	0.696

^aFor linear peptides, N-terminal residues AEGEF are phage coat protein residues. Constrained peptides contain a phage coat protein G residue at their C-terminus.

^bNumber of IgY samples that the peptides was enriched against/total number of IgY samples: enrichment was defined as peptides giving a Z score of ≥2.0.

^cROC curves were produced for the recognition of the peptides with IgY from S. Enteritidis infected (n = 19) and S. Typhimurium or S. Hadar infected (n = 25) chickens in an ELISA. AUC and corresponding p values are listed.