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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: J Immunol. 2010 Jun 25;185(3):1836–1845. doi: 10.4049/jimmunol.1000021

FIGURE 3.

FIGURE 3

Obesity reduces naive and expands E/M T cells in adipose depots. The frequency of CD4 (A) and CD8 E/M (CD62L−CD44+) (B) cells in spleen, eFat (V-Fat), and inguinal fat depot (S-Fat) of 9-mo-old lean (chow fed) and DIO mice (n = 6–9). The frequency of CD4 (C) and CD8 (D) naive (CD62L+CD44−) cells in spleen and adipose depots of 9-mo chow and DIO mice. E, Distribution of CD4 and CD8 naive and E/M T cells in perirenal fat of 5- to 6-mo-old chow and DIO male mice (n = 6). The forward light scatter/side scatter (of light) gate represents location of T cells in SVF cells in collagenase-digested murine adipose tissue. F, The representative FACS plot depicting the frequency of T cells in the liver of 5-mo-old chow and DIO male mice. Because of the few T cells in liver, CD4- and CD8-gated T cells did not present sufficient events to discriminate naive and E/M populations. G, The naive and E/M T cell distribution in perirenal fat. All data are represented as mean (SEM); *p<0.05, statistical significance. H, The CD4+ ARTs from epididymal (V-Fat), inguinal fat (S-Fat), and spleen of 9-mo-old lean and DIO mice were purified by FACS sorting and used to prepare DNA and signal-joint TREC levels were analyzed using quantitative PCR analysis. S-Fat, s.c.-fat; V-Fat, visceral-fat.