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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: J Immunol. 2010 Jun 25;185(3):1836–1845. doi: 10.4049/jimmunol.1000021

FIGURE 4.

FIGURE 4

Obesity restricts TCR diversity of ART cells. A, The CD4 cells were sorted from spleen and VAT of chow-fed and high-fat fed DIO mice (13 mo old) to prepare cDNA that was used for TCR spectratyping. The three-dimensional graph depicts perturbation in CD4 ARTs in VAT compared with spleen. Each line crossing on the y-axis of the landscape denotes change from splenic CD4 cell-specific CDR3 length or size (x-axis) of a particular Vβ family (z-axis). The perturbations in TCR diversity are shown as landscape surfaces, in which smooth (blue) landscapes show an unchanged TCR diversity. The mountains (in green, yellow, and orange) depict area under the curve of amplified peaks of CDR3 lengths. B and C, Effect of obesity on the CDR3 lengths of altered Vβ families of CD4 and CD8 ARTs in SAT and VAT. The data are representative of pooled ARTs from a total of 12 mice.