Figure 1.

Promoter single nucleotide polymorphisms (SNPs) affect IL-6 secretion in fibroblast cells treated with H₂O₂ and benzo[a]pyrene diolepoxide (BPDE). Abbreviations: DMEM, Dulbecco’s modified essential medium; FBS, fetal bovine serum. Six fibroblast cell lines, three wild homozygotes (GG), and three heterozygotes (AG) for rs1800797 were treated with H₂O₂ and BPDE. The height of the bar is the average concentration of IL-6 in culture medium that is also expressed as the number under the figure. The error bar is the standard deviation from three independent experiments. A generalized linear model was used to assess the effects of H₂O₂ or BPDE treatment and rs1800797 genotype on IL-6 concentration detected in the medium. A strong IL-6 induction was identified for both carcinogens (p-values < 0.0001). The slope for the induction of IL-6 secretion by H₂O₂ or BPDE treatment is 74% and 39% greater in AG lines than GG lines (p-values = 0.017 for H₂O₂ and 0.13 for BPDE).