Fig. 10.

Accumulation of abnormal OsUDT1 transcripts in OsRH2 and OsRH34 double-knockdown plants. a Illustration of gene structure and abnormal transcript structures of OsUDT1 [24], and the positions of primers used for RT-PCR analysis. Gray rectangles, UTRs; white rectangles, exons (E); lines, introns (I). b and c Accumulation of OsUDT1 abnormal transcripts. Total RNAs were isolated from inflorescence of WT, RH2Ri 2b, RH2Ri 4, and RH2Ri 14b plants at vegetative stage. Unspliced OsUDT1 pre-mRNAs were detected by RT-PCR analysis with specific primers (Additional file 1). Act1 mRNAs were used as internal control. c Relative level of abnormal (type I, II and III) and mature OsUDT1 mRNAs were determined by Image J with normalization relative to the WT. Error bars indicated the SD of four replicate experiments with two biological replicates. Level of DNA fragment was related to wild-type plants, as 1. * is significantly different from the wild-type plants (Student’s t test: p <0.05)