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. 2016 Apr 12;16:84. doi: 10.1186/s12870-016-0769-5

Fig. 8.

Fig. 8

Seed setting rate and seed development in OsRH2 and OsRH34 double-knockdown transgenic rice. a and b A low seed setting rate was observed in OsRH2 and OsRH34 double-knockdown plants. a Spikelet phenotype of three independent OsRH2 and OsRH34 double-knockdown lines, RH2Ri 2b, RH2Ri 4, and RH2Ri 14b. Bars = 5 cm. b Determination of the numbers of mature and aborted seeds in OsRH2 and OsRH34 double-knockdown lines. Error bars show ± SD (n = 20), * is significantly different from the wild-type plants (Student’s t test: p <0.05). c The OsRH2 and OsRH34 double-knockdown lines showed defects in pollen development. Bars = 200 μm. d–f The OsRH2 and OsRH34 double-knockdown lines showed defects in embryonic development. d Micrographs of husked of wild-type rice seeds at various developmental stages. Rice seeds were harvested at 1, 3, 7, 14, and 30 days after pollination (DAP). e The internal seed stages of the RH2Ri 2b line at 30 DAP. f Determination of the numbers of the seeds at different stages in the RH2Ri 2b transgenic and wild-type plants