Figure 2. Thermal inactivation of His₆-N-hCPT2 and His₆-N-hCPT2/S113L at different temperatures.

Black squares, blue circles, and red triangles represent values at 30°C, 40°C, and 45°C, respectively. Open symbols show the wild-type recombinant carnitine palmitoyltransferase II and the closed symbols show His₆-N-hCPT2/S113L. Data are presented as time-dependent changes of natural-log–transformed relative activities. The experiments were performed as explained in the Methods section.