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. 2016 Mar 3;2(2):e54. doi: 10.1212/NXG.0000000000000054

Figure 1. DNA variations of COQ2 gene in patients with multiple system atrophy.

Figure 1

(A–C) Direct DNA sequencings detect G21S (A), L25V (B), and X422K (C) variations. Arrows point out the position of each variation. (D) G21S DNA variation confirmed by PCR restriction fragment length polymorphism (RFLP) with the arrow as normal band and the arrowhead as variant band. (E) L25V DNA variation confirmed by allele-specific (AS)-PCR with the variant case positive both for common forward (CF) plus normal reverse (NR) primers and CF plus variant reverse (VR) primers. (F) X422K DNA variation confirmed by PCR-RFLP with the variant allele (arrowhead).