Figure 2.

Identification of the binding site of miR-199a in the 3′UTR of CTGF. (A) CTGF was identified as target of miR-199a-5b, with its possible binding region in CTGF 3′UTR. (B) Relative luciferase activity of cells treated with constructs containing CTGF 3′UTR segments was significantly lower compared with the negative controls, while the cells transfected with constructs containing CTGF mutant 3′UTR segments was comparable compared with the negative controls (** P<0.01).