Table 1. Analysis of similarity of seed-associated microbial assemblages.

Analysis of similarity (ANOSIM) was used to assess the robustness of the hierarchical clustering analyses (Bray–Curtis dissimilarity measure and unweighted Unifrac distance). P-values are displayed in each column. Only P-values highlighted in bold are considered as significant.

Marker	Diversity index	OTU	A13vsC13	X13vsC13	A14vsC14	X14vsC14	Manual vs. mechanical
16S rRNA gene	Bray–Curtis	OTUs	0.693	0.249	0.932	0.045	0.991
		aOTUs	0.733	0.223	0.918	0.058	0.989
	Unifrac unweighted	OTUs	0.698	0.298	0.032	0.029	0.976
		aOTUs	0.737	0.407	0.044	0.018	0.962
gyrB	Bray–Curtis	OTUs	0.257	0.208	0.098	0.105	0.999
		aOTUs	0.156	0.138	0.041	0.028	0.998
	Unifrac unweighted	OTUs	0.241	0.520	0.134	0.804	0.999
		aOTUs	0.203	0.467	0.026	0.111	0.957
ITS1	Bray–Curtis	OTUs	<0.001	0.763	<0.001	0.504	0.999
		aOTUs	<0.001	0.777	<0.001	0.406	0.999