Bacterial 16S rRNA |
27f1492r |
AGAGTTTGGATCMTGGCTCAGGYYACCTTGTTACGACTT |
Lane (1991) Nicol et al. (2008) |
95°C 5 min; 10 cycles of 94°C 30 s, 55°C 30 s, 72°C 2 min; 25 cycles of 92°C 30 s, 55°C 30 s, 72°C 2 min; 72°C 5 min |
Fungal 18S rRNA |
Fung5fFF390r |
GGGAACCAGGACTTTTACGAGGTCTCGTTCGTTATCG |
Smit et al. (1999) Vainio and Hantula (2000) |
95°C 5 min; 10 cycles of 94°C 30 s, 55°C 30 s, 72°C 50 s; 25 cycles of 92°C 30 s, 55°C 30 s, 72°C 50 s; 72°C 5 min |
Archaeal 16S rRNA |
A109F 1492r |
ACKGCTCAGTAACACGT GYYACCTTGTTACGACTT |
Grosskopf, Janssen and Liesack (1998) Nicol et al. (2008) |
95°C 5 min; 10 cycles of 94°C 30 s, 55°C 30 s, 72°C 2 min; 25 cycles of 92°C 30 s, 55°C 30 s, 72°C 2 min; 72°C 5 min |
Thaumarchaeal amoA
|
CrenamoA23fCrenamoA616r |
ATGGTCTGGCTWAGACGGCCATCCATCTGTATGTCCA |
Tourna et al. (2008) Tourna et al. (2008) |
95°C 5 min; 10 cycles of 94°C 30 s, 55°C 30 s, 72°C 1 min; 25 cycles of 92°C 30 s, 55°C 30 s, 72°C 1 min; 72°C 5 min |
Nitrosotalea sp. Nd2 amoA gene cluster |
Nd2F1Nd2R2 |
GATACTTGCAGTGATACCTACCCCAGATATTCTTGTTTCAACAGAGG |
This studyThis study |
95°C 5 min; 35 cycles of 94°C 30 s, 52°C 30 s, 72°C 4 min; 72°C 10 min |