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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: ACS Chem Neurosci. 2015 May 1;6(8):1411–1419. doi: 10.1021/acschemneuro.5b00092

Figure 2.

Figure 2

Triazole analogues stimulate G protein coupling, βarrestin2 recruitment, and ERK1/2 phosphorylation to different degrees relative to U69,593. G protein coupling (A) identifies triazole analogues as potent, full agonists in the presence of [35S]GTPγS. High content imaging of βarrestin2 (B) illustrates that triazole analogues recruit βarrestin2 only at concentrations significantly higher than U69,593. ERK1/2 phosphorylation (C) measured by fluorescence intensity detection of the ratio of pERK1/2 to tERK1/2 concludes that triazole test compounds exceeds stimulation observed for U69,593. Assays were run with concentrations of indicated agonists and are displayed as percentage of maximal U69,593 stimulation. Calculated potencies and efficacies are presented in Table 1. Data are presented as the mean ± SEM (n ≥ 3).