Table 2.
entry | ΔLogR
|
ΔΔLogR(assay1−assay2)
|
bias factor (10ΔΔLogR)
|
||||||
---|---|---|---|---|---|---|---|---|---|
G protein assay | βarrestin2 assay | ERK 1/2 | ΔΔLogR(G−βarr2) | 95% CI | ΔΔLogR(G−ERK) | 95% CI | G/βarr2 | G/ERK | |
U69,593 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
1.1 | 0.28 ± 0.16 | −1.39 ± 0.11 | −1.69 ± 0.26 | 1.7 ± 0.19 | 1.2–2.1 | 2.0 ± 0.19 | 1.5–2.4 | 47 | 93 |
1.2 | 0.59 ± 0.12 | −1.50 ± 0.21 | −1.93 ± 0.18 | 1.9 ± 0.21 | 1.6–2.5 | 2.6 ± 0.19 | 2.2–3.1 | 122 | 419 |
1.3 | −0.34 ± 0.09 | −1.50 ± 0.15 | −1.29 ± 0.06 | 1.2 ± 0.20 | 0.7–1.6 | 1.0 ± 0.19 | 0.5–1.4 | 15 | 9 |
2.1 | 0.30 ± 0.13 | −1.39 ± 0.13 | −1.64 ± 0.10 | 1.7 ± 0.19 | 1.3–2.1 | 1.9 ± 0.19 | 1.5–2.4 | 49 | 88 |
2.2 | 0.33 ± 0.19 | −1.19 ± 0.10 | −1.97 ± 0.20 | 1.5 ± 0.19 | 1.1–2.0 | 2.5 ± 0.19 | 1.9–3.0 | 33 | 304 |
2.3 | 0.01 ± 0.12 | −1.40 ± 0.20 | −1.21 ± 0.01 | 1.4 ± 0.22 | 0.9–1.9 | 1.2 ± 0.22 | 0.7–1.7 | 25 | 16 |
3.1 | −0.34 ± 0.16 | −1.58 ± 0.11 | −1.93 ± 0.09 | 1.2 ± 0.20 | 0.8–1.7 | 1.7 ± 0.20 | 1.2–2.1 | 17 | 47 |
3.2 | −0.24 ± 0.17 | −1.09 ± 0.09 | −1.82 ± 0.10 | 0.9 ± 0.22 | 0.4–1.3 | 1.7 ± 0.22 | 1.2–2.2 | 7 | 46 |
3.3 | −0.55 ± 0.14 | −2.12 ± 0.16 | −1.63 ± 0.06 | 1.6 ± 0.22 | 1.1–2.1 | 1.1 ± 0.19 | 0.7–1.6 | 37 | 14 |
The parameter estimates are presented as mean ± SEM (n ≥ 3). The ΔΔLogR for each bias calculation is presented ± SEM (n ≥ 3) along with 95% confidence interval (95% CI). Bias factor is calculated as the antilog of the ΔΔLogR for each response pair.