. 2016 Apr 13;3:16017. doi: 10.1038/mtm.2016.17

Table 3. Standard downstream process technologies applicable to LV purification and recovery.

Purpose of downstream process steps	Technologies used for LV downstream processing
Removal of cells and debris	Frontal filtration 0.45µm⁴²
Removal of cells and debris	Centrifugation⁹⁹
Capture chromatography	Anion-exchange chromatography (Mustang Q^{42,52,100,102,105} or DEAE Sepharose^52,55,56)
Capture chromatography	Affinity chromatography (heparin)¹⁰⁶
Polishing	Size-exclusion chromatography^{42,52,105,110}
Concentration and buffer exchange	Tangential flow filtration⁴²
Concentration and buffer exchange	Ultracentrifugation⁹⁹
DNA reduction	Benzonase^{42,52,54,55,61,105}
Sterilization	0.2-µm filter¹¹³

LV, lentiviral vectors.