Table 4. Results of large-scale downstream processing of VSV-g-pseudotyped LV vectors.
Company/institution | Concentration factor (x) | Overall yield (%) | Final titer |
---|---|---|---|
Beckman Research Institute61 | 150–200 (use of ultracentrifugation) | 40 | 2.6–3.8 × 108 TU/ml |
Virxsys52 | 20 | 30 | 2.17 × 108 TU/ml |
Généthon42/MolMed105 | 50/100 | 20 | 1–2 × 109 IG/ml |
Oxford Biomedica/Henogen (Mitrophanous, personal communication) | 2,000 (use of two succeeding TFF steps) | 30–40 | 0.1–2 × 109 TU/ml |
St. Jude Children’s Hospital56 | 65–80 | 29–33 | 4.5–7.2 × 108 TU/ml |
LV, lentiviral vectors; VSV-g, glycoprotein of the vesicular stomatitis virus.