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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: Mamm Genome. 2015 May 1;26(5-6):264–270. doi: 10.1007/s00335-015-9564-0

Figure 1.

Figure 1

Comparison of iNKT cells between CD1d KO and control pigs. (A) Frequency of peripheral blood iNKT cells. Left panel describes the gating strategy to identify iNKT cells. Single cell suspensions were membrane-labeled with anti-CD3ε mAb and PBS57-loaded CD1d tetramer. Some samples were also labeled with an unloaded CD1d tetramer to validate the staining of PBS57-loaded CD1d tetramer. Right panel describes the frequency of iNKT cells as a proportion of CD3+ cells. For controls, samples were collected from 6 NSRRC pigs (closed circles) and 9 UF pigs (open circles) (B) iNKT cell responses to α-GalCer as measured by IFNγ-ELISPOT assay in which PBMCs were incubated for 72 h with 1 μg/ml α-GalCer. Results represent mean IFNγ spots per 1×105 PBMCs after subtracting spots in unstimulated samples.