Table 1.
Reagents used for flow cytometry analysis of surface markers and intracellular FoxP3 and CD79α
| Antigen | Clone | Isotype | Fluorochrome conjugation |
Source |
|---|---|---|---|---|
| CD1d tetramer | N/A | N/A | PE | NIH Tetramer Core Facility |
| Anti-CD3ε | BB23-8E6-8C8 | Mouse IgG2aκ | FITC/PE | BD Biosciences |
| Anti-CD8 | 76-2-11 | Mouse IgG2aκ | PE | BD Biosciences |
| Anti-CD4 | 74-12-4 | Mouse IgG2bκ | Alexa647 | Southern Biotech |
| Anti-FoxP3 | FJK-16s | Rat IgG2aκ | PE | eBioscience |
| Anti-TCRδ | PGBL22A | Mouse IgG1 | Alexa647 | Washington State University |
| Anti-CD172α | 74-22-15A | Mouse IgG2bκ | Alexa488 | BD Biosciences |
| Anti-CD79α | HM47 | Mouse IgG1κ | PE | BD Biosciences |
| Anti-MHC class II | H42A | Mouse IgG2a | Alexa647 | Accurate |