Fig. 3.
rGrnB is an IDP. (A) Concentration-dependent conformational changes in rGrnB observed by far-UV CD. (Inset) Spectra as a difference in the molar ellipticities at 222 nm (helix) and 200 nm (random coil). (B) Fractionation of rGrnB using a Superdex™ 75 size exclusion column (solid line) and compared with the fractionation profile of gel filtration standards (dotted line)—1-bovine thyroglobulin (670 kDa), 2-bovine γ-globulin (158 kDa), 3-chicken ovalbumin (44 kDa), 4-horse myoglobin (17 kDa) and 5-vitamin B12 (1.35 kDa).