Table 1.
Characterization of reconstructed single, double and triple mutants
| Strain | Mutated gene(s) | MIC (mg/L) |
Mean ± SD relative growth rate | Mean ± SD relative expressiona |
Reference | |||
|---|---|---|---|---|---|---|---|---|
| TGC | TET | marA | acrA | acrB | ||||
| DA5438 | — | 0.047 | 1 | 1 | 1 | 1 | 1 | 5 |
| DA25822 | lon Q137stop | 0.125 | 1 | 0.96 ± 0.04 | 0.8 ± 0.2 | 1.6 ± 0.3 | 1.6 ± 0.4 | 5 |
| DA25825 | marR K62fs | 0.125 | 1.5 | 0.97 ± 0.03 | 47.0 ± 3.6 | 5.0 ± 0.5 | 3.8 ± 0.5 | 5 |
| DA26800 | lpcA E173G | 0.125 | 1.5 | 0.91 ± 0.01 | 1.7 ± 0.4 | 1.1 ± 0.2 | 1.1 ± 0.3 | 5 |
| DA40498 | lpcA E173G, lon Q137stop | 0.19 | 1 | 0.75 ± 0.02 | 3.3 ± 0.7 | 5.7 ± 1.4 | 4.4 ± 0.6 | this study |
| DA39146 | lpcA E173G, marR K62fs | 0.25 | 2 | 0.79 ± 0.01 | 88.8 ± 6.1 | 6.8 ± 0.2 | 4.7 ± 0.3 | this study |
| DA39150 | lon Q137stop, marR K62fs | 0.75 | 6 | 0.68 ± 0.01 | 100.7 ± 5.4 | 10.8 ± 0.8 | 7.5 ± 0.8 | this study |
| DA39997 | lpcA E173G, lon Q137stop, marR K62fs | 0.25 | 2 | 0.76 ± 0.01 | 2.8 ± 0.3 | 2.6 ± 0.2 | 1.9 ± 0.2 | this study |
TGC, tigecycline; TET, tetracycline; fs, frame shift; stop, stop codon.
aExpression level measurements.