Fig. 2.
Relaxant effect of [Ca2 +]o on pre-contracted tone involve CaSRs. (A) Mean data showing that relaxations of pre-contracted tone induced by increasing [Ca2 +]o from 1 mM to 6 mM are reduced by pre-treatment with 3 μM Calhex-231. Each point is from n = 4 animals, with at least n = 3 segments per animal. **p < 0.01, ***p < 0.001. (B) Immunocytochemistry images showing that CaSRs are expressed in ECs and VSMCs. PECAM-1 and α-actin are selective markers for ECs and VSMCs respectively. DAPI stains the cell nucleus.