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Effect of NO–GC–PKG pathway inhibitors on [Ca2 +]o-induced relaxations of pre-contracted tone. [Ca2 +]o-induced relaxations of pre-contracted tone were markedly inhibited by (A) the eNOS inhibitor L-NAME (300 μM), (B) the guanylate cyclase inhibitor ODQ (10 μM) and (C) the PKG inhibitor KT5823 (1 μM). Each point is from n = 4 animals, with at least n = 3 segments per animal. **p < 0.01, ***p < 0.001.
