Figure 2.
Co-culture of DRG explants, SCs and HAECs with control or VEGF-MSCs increased neurite outgrowth and cell proliferation. (A) Neurite length was increased in DRG explants in co-cultures with control and VEGF-MSCs (*p<0.01 versus basal media). (B) SC proliferation was increased in co-cultures with control and VEGF-MSCs (*p<0.01 versus basal media). (C) HAEC proliferation was increased in co-cultures with control and VEGF-MSCs compared to basal media, and co-cultures with VEGF-MSCs increased proliferation compared to control MSCs (*p<0.01, n≥6). (D–F) Supplementation of cultures with human recombinant VEGF increased cell proliferation in (F) HAEC cultures (*p<0.01) but not (D) DRG explants or (E) SC cultures (n≥7). Data are normalized to their respective basal media controls. Data are mean ± SEM.