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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: Anal Chem. 2015 Apr 7;87(8):4159–4167. doi: 10.1021/acs.analchem.5b00589

Figure 3.

Figure 3

Experimental results of polystyrene bead sorting. (a) Fluorescence microscopy image overlay (100 frames) of 500 nm and 2.5 μm bead sorting showing the microbeads (thick lines) focusing into “C” while the nanobeads (thin lines) deflect into “S” at the model-optimized potential scheme (VI = +200 V, VS = −155 V, VC = −750 V). (b) Fluorescence microscopy image overlay (100 frames) of 2.5 μm beads focusing into “C” while (c) shows 200 nm beads deflecting into “S” under lower applied potentials (VI = +200 V, VS = −155 V, VC = −250 V). (d) Calculated Σ for both sorting events indicating high levels of sorting (>90%) in both cases.