Table 3. Top 20 most highly expressed novel miRNAs identified in the 10 mothers studied in both cell and lipid HM fractions and across the three lactation stages examined (months 2, 4 and 6), with the total reads, and the number of samples that each miRNA was detected in (total samples n = 45).

miRNA species	Mature sequence	Total reads	Nucleotide length (nt)	Number of samples determined in (out of n = 45)
novel_mir_189	GCCTGTCTGAGCGTCGCT	751,632	18	13
novel_mir_2	ATGTTGGATCAGGACATCC	18,054	19	20
novel_mir_112	GACCTCGCCGTCCCGCCCGCC	5,608	21	8
novel_mir_4	TCCATATCCCAACCTGTCAGAGT	5,581	23	27
novel_mir_5	AATGTGGCTTAGAACATG	3,495	18	27
novel_mir_392	GCGCGCCCCCGCCCCGGC	1,520	18	14
novel_mir_472	TAGACGGGCTCACATCACC	1,288	19	10
novel_mir_1905	TTAGGTCAAGGTGTAGCC	1,242	18	2
novel_mir_471	GACCTCGCCGTCCCGCCCGCCG	1,200	22	2
novel_mir_2797	GTCGGGGCGGCGGCGGCGGCG	1,104	21	2
novel_mir_4933	GTTAGGTCAAGGTGTAGCC	1,080	19	1
novel_mir_1906	GCGGCGGCGGCGGCGGGACCG	927	21	2
novel_mir_1681	TACGGATCTGGCTTCTGAGA	795	20	4
novel_mir_397	TTTAGACGGGCTCACATCACC	766	21	7
novel_mir_3593	GCGGCGGCGGCGGCGGCGGGGC	756	22	1
novel_mir_3	TCTGCAGGCTGGGATCTGGGAT	753	22	17
novel_mir_110	TCTGCAGGCTGGGATCTGGGA	657	21	17
novel_mir_113	ACTAGGATTGTGCTTCCCTGG	622	21	23
novel_mir_197	TGCACTACAGAACTTTGA	592	18	24
novel_mir_116	TGTAAACATCCTTGACTGA	578	19	23