Fig 6. Targeting OVA-antigen to Clec9A on neonatal CD8α^- DCs induces protection and efficient CD8⁺ T-cell response to Lm infection.

A, Immunization protocol for B to D. B, Survival of vaccinated mice, followed during 15 days (n = 12-15/group). C, 3 days after Lm-OVA challenge, spleens were harvested and Listeria burden was quantified (n = 4/group). D, 5 days post Lm-OVA challenge, spleen cells of indicated treated-mice were collected and cultured with IL-2 (10ng/mL) and OVA^257-264. CD8⁺CD3⁺ cells were analyzed for IFN-γ production by intracellular staining (n = 3-4/group).