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. 2016 Apr 13;11(4):e0153434. doi: 10.1371/journal.pone.0153434

Fig 4. Renal collagen deposition in offspring of prenatal LPS exposure could be reversed by post-natal NF-κB inhibition.

Fig 4

(A) Level of renal collagen deposition in 68-week-old offspring was assessed by Sirius red staining and observed under both white microscope (top panel) and polarized microscope (Arrow indicates collagen III accumulation, bottom panel). Semi-quantitation of relative Sirius red-positive area was shown (B). Relative mRNA expressions of collagen type1 (Col1a1) and collagen type 3 (Col3a1) (C) were determined by realtime RT-PCR in kidney at the age of 20 weeks. Data are presented as mean ± SD. n = 7 offspring and 4–5 pictures from each offspring were quantified for (B). n = 7 offspring in each group for (C). * and ** indicate P<0.05 and P<0.01, respectively, which denote statistical comparison between the two marked treatment groups (Two-way ANOVA followed by Dunnett T3 test (B) or LSD test (C) for inter-group comparison). Indications of Con+Ve, LPS+Ve, LPS+PDTC and Con+PDTC are as described in Fig 1.