Fig 9. Oxidation conditions in WT and TaEXPA2 transgenic plants under drought stress.

Changes in MDA content (A) and relative electrical conductivity (B) in WT and transgenic tobacco leaves under drought stress. (C) In situ detection of H₂O₂ and O₂⁻ by DAB staining (upper) and NBT staining (bottom) in WT and TaEXPA2 transgenic seedlings grown on normal medium for 14 days and then treated with 100 mM mannitol for 7 days. The experiment was replicated three times. (D) H₂O₂ content and (E) O₂⁻ production rate in 14-day-old plants treated with 100 mM mannitol for 7 days. Each column represents an average of three replicates, and bars indicate SEs. * and ** indicate significant differences of P < 0.05 and P < 0.01, respectively, according to Duncan’s multiple range test.