Table 1. Analyses of participants’ concentration procedures.
| Analyses of participants’ procedures | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Preservative * | Solvent ** | |||||||||||
| Manufacturer | Manufacturer’s instructions | FW | FS | SAF | PVA | MIF | Ecofix | Et | EtA | EtA +TrX | None | No of participants who followed manufacturer’s procedure for centrifugal speed and time |
| Modified Ridley-Allen N = 57 | 10% formalin/water, Ether as solvent, Centrifuge at 3000 rpm for 1 min. | 21 | 10 | 16 | 2 | 1 | 19 | 25 | 2 | 6 (for the remainder, centrifugal speed ranged from 500–3,500 rpm and time from 1–10 mins | ||
| Midi Parasep N = 59 | 10% formalin/water, Ethyl acetate/Triton X, Centrifuge at 3000 rpm for 1–3 minutes | 15 | 27 | 10 | 2 | 23 | 24 | 22 (for the remainder, centrifugal speed ranged from 500–3,000 rpm and time from 1–5 mins) | ||||
| Midi Parasep SF N = 24 | 10% formalin/water, SAF,PVA,MIF; No solvent; Centrifuge for 1,400 rpm for 3 minutes | 8 | 7 | 9 | 24 | 14 (for the remainder, centrifugal speed ranged from 500–3000 rpm and time from 1–3 mins) | ||||||
| Mini Parasep SF N = 3 | 10% formalin/water,SAF,PVA,MIF; No solvent; Centrifuge for 200g for 3 minutes | 3 | 3 | Manufacturer’s instructions not followed (Centrifugal speed ranged from 1,200–2,500 rpm and time from 1–2 mins) | ||||||||
| FPC Evergreen N = 17 | 10% formalin/water, Ethyl acetate, Centrifuge at 500g for 10 mins | 5 | 4 | 8 | 5 | 3 | 2 | 1 | 4 (for the remainder, centrifugal speed ranged from 1,200–2,500 rpm and time from 1–10 mins) | |||
| Parapak N = 15 | Formalin/water, PVA, SAF or Ecofix, Ethyl acetate, 1,800–2,200 rpm for 10 mins | 4 | 1 | 5 | 1 | 1 | 1 | 9 | 1 | 2 | 5 (for the remainder, centrifugal speed ranged from 1,200–3,000 rpm and time from 1–5 mins) | |
| Diamondial N = 8 | Formalin/water, PVA, SAF or Ecofix, Ethyl acetate, 1,000g for 10 mins | 2 | 4 | 4 (for the remainder, centrifugal speed ranged from 3,000–3,500 rpm and time from 1–5 mins) | ||||||||
| Easy Copros N = 2 | Formalin/water, ethyl acetate, 1,500rpm for 5 mins. | 1 | 1 | 1 participant did not state speed and time the other centrifuged at 1,500 rpm for 10 mins. | ||||||||
| Storax N = 1 | Manufacturer’s instructions not found | 1 | 1 | Participant centrifuged at 1,800 rpm for 10 mins. | ||||||||
| Para-Sed MCC N = 1 | Formalin/water, SAF,ethyl acetate/triton X, 500g for 10 mins | 1 | 1 | Participant followed manufacturer’s instructions | ||||||||
| Biosepar | Medium FixSepar ECO, 1000g for 5 minute | 1 | 1 | Participant centrifuged at 1,500 rpm for 2 mins | ||||||||
| In-house N = 8 | 2 | 2 | 3 | 1 | 3 | Centrifugal speeds ranged from 500–3,000 rpm and time 1–10 mins. | ||||||
| No concentration method N = 7 | Direct Examination only | |||||||||||
*The preservatives used are as follows: FW, 10% formalin in water; FS, 10% formalin in saline; SAF, Sodium acetic acid acetate formalin; MIF merthiolate Iodine formalin; PVA Poly vinyl alcohol.
** The solvents used are as follows: Et, ether; EtA, ethyl acetate; EtA TrX, ethyl acetate + TritonX
Numbers of participants using solvent and preservative do not equate to the total number of participants using the particular test because many participants did not state what preservative or solvent they used.