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. 2015 Sep 1;135(1):50–59. doi: 10.1111/jnc.13255

Figure 2.

Figure 2

Cellular localization and subcellular distribution of selected transcripts determined by in situ hybridization histochemistry. (a, b) Robust expression of dopamine (DA) transporter mRNA within the processes and soma of DA neurons (also readily identifiable by their characteristic large nuclei and high intracellular neuromelanin content). (c, d) Specificity was demonstrated by the absence of signaling using a riboprobe derived from bacterial neomycin gene sequence as a negative control. (e, f) Similar to DA transporter mRNA localization, LINC00162 transcript was robustly expressed within the processes and soma of DA cells, with nuclear exclusion. (g, h) TRAF3IP2‐AS1 transcript exhibited a strong nuclear localization in DA cells. (i, j) TRAF3IP2 protein‐coding transcript distribution was distinctly different from TRAF3IP2‐AS1 transcript, and was found throughout the nucleus, cytoplasm and processes of DA neurons. Probe sequences can be found in Table S1. Images captured with a 60× objective. Scale bars equal 10 microns.