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. 2016 Mar 31;2016:8382605. doi: 10.1155/2016/8382605

Table 1.

Primer name, primer sequences, and expected amplicon size of amplified DNA products.

PCR name β-lactamase targeted Primer name Sequence (5′-3′) Amplicon size (bp)
VIM VIM variants including VIM-1 and VIM-2 VIM-for GATGGTGTTTGGTCGCATA 390
VIM-rev CGAATGCGCAGCACCAG

TEM TEM variants including TEM-1 and TEM-2 TSO-T-for CATTTCCGTGTCGCCCTTATTC 800
TSO-T-rev CGTTCATCCATAGTTGCCTGAC

SHV SHV variants including SHV-1 TSO-S-for AGCCGCTTGAGCAAATTAAAC 713
TSO-S-rev ATCCCGCAGATAAATCACCAC

CTX-M group 1 Variants of CTX-M group 1 including CTX-M-1, CTX-M-3, and CTX-M-15 CTXMGp 1-for TTAGGAARTGTGCCGCTGYAb 688
CTXMGp 1.2 rev CGATATCGTTGGTGGTRCCATb

CTX-M group 9 Variants of CTX-M-9 including CTX-M-9 and CTX-M-14 CTX-9-F TCAAGCCTGCCGATCTGGT 561
CTX-9-R TGATTCTCGCCGCTGAAG

CTX-M group 8/25 CTX-M-8, CTX-M-25, CTX-M-26, and CTX-M-39 to CTX-M-41 CTX-8/25-F AACRCRCAGACGCTCTACb 326
CTX-8/25-R TCGAGCCGGAASGTGTYATb

bY = T or C; R = A or G; S = G or C.