Table 1.
PCR name | β-lactamase targeted | Primer name | Sequence (5′-3′) | Amplicon size (bp) |
---|---|---|---|---|
VIM | VIM variants including VIM-1 and VIM-2 | VIM-for | GATGGTGTTTGGTCGCATA | 390 |
VIM-rev | CGAATGCGCAGCACCAG | |||
| ||||
TEM | TEM variants including TEM-1 and TEM-2 | TSO-T-for | CATTTCCGTGTCGCCCTTATTC | 800 |
TSO-T-rev | CGTTCATCCATAGTTGCCTGAC | |||
| ||||
SHV | SHV variants including SHV-1 | TSO-S-for | AGCCGCTTGAGCAAATTAAAC | 713 |
TSO-S-rev | ATCCCGCAGATAAATCACCAC | |||
| ||||
CTX-M group 1 | Variants of CTX-M group 1 including CTX-M-1, CTX-M-3, and CTX-M-15 | CTXMGp 1-for | TTAGGAARTGTGCCGCTGYAb | 688 |
CTXMGp 1.2 rev | CGATATCGTTGGTGGTRCCATb | |||
| ||||
CTX-M group 9 | Variants of CTX-M-9 including CTX-M-9 and CTX-M-14 | CTX-9-F | TCAAGCCTGCCGATCTGGT | 561 |
CTX-9-R | TGATTCTCGCCGCTGAAG | |||
| ||||
CTX-M group 8/25 | CTX-M-8, CTX-M-25, CTX-M-26, and CTX-M-39 to CTX-M-41 | CTX-8/25-F | AACRCRCAGACGCTCTACb | 326 |
CTX-8/25-R | TCGAGCCGGAASGTGTYATb |
bY = T or C; R = A or G; S = G or C.