Table 1.
Gene | Product size (bp) | Primer sequence |
---|---|---|
Calpain 2 | 165 | F: 5′-CTGGGGCTGAAGGAGTTCTAC |
R: 5′-GATGACTTGGTGGAGTTGACAG | ||
| ||
CnA α isoform | 112 | F: 5′-CAGGAACATTTCACTCACAACACAG |
R: 5′-CGTGGGCTCGGAGTATAGATAACA | ||
| ||
CnA β isoform | 119 | F: 5′-TGGATGTCTTCACGTGGTCTTTAC |
R: 5′-ATCAAACTGGTCTTCACCTTCAGTC | ||
| ||
NFAT-c3 | 137 | F: 5′-CATCGAGCCCATTATGAAACTGAA |
R: 5′-CGATCATCTGCTGTCCCAATAAAC | ||
| ||
NFAT-c4 | 97 | F: 5′-AGCCTGACACACCGTAGGTACTGA |
R: 5′-AGTGCAAATGCCCGGAATG | ||
| ||
β-actin | 366 | F: 5′-ACACTGTGCCCATCTACGAGGGG |
R: 5′-ATGAGTGAGTTGAAGGTAGTTTCGTGGAT |
CnA, subunit A of calcineurin; F, forward primer; NFAT, nuclear factor of activated T cells; R, reverse primer.