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. 2016 Mar 31;2016:4639654. doi: 10.1155/2016/4639654

Table 1.

Primer sequences were used in qPCR experiments.

Gene Product size (bp) Primer sequence
Calpain 2 165 F: 5′-CTGGGGCTGAAGGAGTTCTAC
R: 5′-GATGACTTGGTGGAGTTGACAG

CnA α isoform 112 F: 5′-CAGGAACATTTCACTCACAACACAG
R: 5′-CGTGGGCTCGGAGTATAGATAACA

CnA β isoform 119 F: 5′-TGGATGTCTTCACGTGGTCTTTAC
R: 5′-ATCAAACTGGTCTTCACCTTCAGTC

NFAT-c3 137 F: 5′-CATCGAGCCCATTATGAAACTGAA
R: 5′-CGATCATCTGCTGTCCCAATAAAC

NFAT-c4 97 F: 5′-AGCCTGACACACCGTAGGTACTGA
R: 5′-AGTGCAAATGCCCGGAATG

β-actin 366 F: 5′-ACACTGTGCCCATCTACGAGGGG
R: 5′-ATGAGTGAGTTGAAGGTAGTTTCGTGGAT

CnA, subunit A of calcineurin; F, forward primer; NFAT, nuclear factor of activated T cells; R, reverse primer.