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Effects of EA on the expression of Nrf2 after MCAO. (a and b) Immunohistochemistry staining for Nrf2-positive cells in hippocampal CA1 region in different groups (n = 6 animals per group) (×400). (c–e) Analysis of Nrf2, GCSh, and GCSl mRNA expression levels in cortex by real-time PCR. GAPDH was used as an internal control (n = 8 animals per group). Data represent mean ± SEM. ## P < 0.01, and ### P < 0.001 versus Sham and Δ P < 0.05, ΔΔ P < 0.01, and ΔΔΔ P < 0.001 versus MCAO.
