Table 2.
Validation parameters of the UAE-B/LC-MS method
| Ergine | Ergometrine | |||||||
|---|---|---|---|---|---|---|---|---|
| Standard addition (μg g-1) | Standard addition (μg g–1) | |||||||
| +0 | +80 | +160 | +240 | +0 | +10 | +20 | +30 | |
| Recovery (%) | - | 91 ± 11 | 99 ± 12 | 106 ± 8 | - | 102 ± 8 | 104 ± 10 | 109 ± 3 |
| Matrix effect (%) | - | 105 ± 3 | 100 ± 5 | 94 ± 1 | - | 105 ± 4 | 94 ± 3 | 90 ± 3 |
| Repeatability (%) | 8.4 | 4.3 | 5.5 | 6.6 | 6.9 | 3.7 | 6.2 | 6.9 |
| Intermediate precision (%) | 11.8 | 11.8 | 9.4 | 11.0 | 1.1 | 5.0 | 5.5 | 1.1 |
| Linearity (μg g–1)a; (ng mL–1) | 5.0–300 | 3.0–300 | ||||||
| LOD (μg g–1) a; (ng mL–1) | 1.0 | 1.0 | ||||||
| LOQ (μg g–1)a; (ng mL–1) | 3.0 | 3.0 | ||||||
aCalculated with regard to 10 mg seed sample