Fig. 3.

Srxn1-GFP BAC HepG2 reporter cell line is dependent on Nrf2/KEAP1 signaling. a Cell injury assay using Annexin-V-Alexa-633 staining after 24-h exposure to our compound set. b Western blot of Nrf2 expression in HepG2 cells exposed for 8 or 16 h to MEN, di-ethyl maleate (DEM), diclofenac (DCF) or KTZ. Density quantification is relative to actin levels, normalized to DMSO. c Western blot of GFP expression in HepG2 Srxn1-GFP cells as in b. Density quantification below is relative to tubulin levels. d Stills of time-lapse imaging of HepG2 Srxn1-GFP cells exposed to Nrf2 inducers. e Quantification of the Srxn1-GFP reporter response kinetics. f siRNA-mediated knockdown of Nrf2 (+siNrf2) or KEAP1 (siKEAP1) or mock treatment (−) in HepG2 Srxn1-GFP cells exposed to DMSO, MEN, DEM, DCF or KTZ for 24 h