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Step-wise spliceosome assembly from its U-snRNP components. The U1 snRNP crystal structure [16, 17•] and available EM maps of some of the complexes are shown: pre-spliceosome (EMDB entry EMD-1325), U4/U6.U5 tri-snRNP [23••], BΔU1 [27], complex C [70] and intron lariat spliceosome (ILS) [24••].
