Table 1. Sequencing assay for ZFN-induced mutations in the FBN1-targeted region.
Wild type genomic DNA | ||
CAGTCCCTCGACCACCAGTGGAATATCCATATCCGTCTCGG | ||
FBN1ZFN05 binding sequence | ||
CAGTCCCTCGACCACCAGnnnnnTATCCATATCCGTCTCGG | ||
Homozygous mutants | ||
F038 | CAGTCCCTCGACCACCAG*****TATCCATATCCGTCTCGG | 5bp del |
F063 | CAGTCCCTCGACCA***********TCCATATCCGTCTCGG | 11bp del |
F162 | CAGTCCCTCGACCA***************TATCCGTCTCGG | 15bp del |
F007 | CAGTCCCTCGACCACCAGACGGATATCCATATCCGTCTCGG | 4bp sub |
CAGTCCCTCGACCAC*************ATATCCGTCTCGG | 13bp del | |
F022 | CAGTCCCTCGACCACCAG****ATATCCATATCCGTCTCGG | 4bp del |
CAGTCCCTCGACC*****TGGAATATCCATATCCGTCTCGG | 5bp del | |
F083 | CAGTCCCTCGACCAC****GGAATATCCATATCCGTCTCGG | 4bp del |
CAGTCCCTCGACC**************CATATCCGTCTCGG | 14bp del | |
F117 | CAGTCCCTCGACCACCA************TATCCGTCTCGG | 12bp del |
CAGTCCCTCGACCACCAG*************TCCGTCTCGG | 13bp del | |
Heterozygous mutants | ||
F002 | CAGTCCCTCGACCACCA***(111bp del)**TTAAGTCA | 111bp del |
F005 | CAGTCCCTCGACCACCAGTGGATGGAATATCCATATCCGTCTCGG | 4bp ins |
F013 | CAGTCCCTCGACCACCAG***AATATCCATATCCGTCTCGG | 3bp del |
F020 | CAGTCCCTCGACCACCAGT*****ATCCATATCCGTCTCGG | 5bp del |
F029 | CAGTCCCTCGACCA**(63del + 7bp ins)**CTTTTC | 56bp del |
F035 | CAGTCCCTCGACCACCAGTG***TATCCATATCCGTCTCGG | 3bp del |
F036 | CAGTCCCTCGACCACCAGTGGAATGGAATATCCATATCCGTCTCGG | 5bp ins |
F041 | CAGTCCCTCGACCACCAG****ATATCCATATCCGTCTCGG | 4bp del |
F043 | CAGTCCCTCGACCACCA******TATCCATATCCGTCTCGG | 6bp del |
F047 | CAGTCCCTCGACCACCAGTG*AATATCCATATCCGTCTCGG | 1bp del |
F050 | CAGTCCCTCGACCACCAG**GAATATCCATATCCGTCTCGG | 2bp del |
F074 | CAGTCCCTCGACCACCAGTGGAA*ATCCATATCCGTCTCGG | 1bp del |
F098 | CAGTCCCTCGACCACCAG****ATATCCATATCCGTCTCGG | 4bp del |
Multiple deletions or insertions depicted using asterisks or underlines, respectively.