Table 2. In vitro development of SCNT embryos and production of heterozygous FBN1 mutant cloned piglets.
In vitro development of SCNT embryos | ||||
SCNT embryos reconstructed | 395 | 334 | ||
Early cleavage-stage embryos | 302 | 272b | ||
Blastocyst-stage embryos on day 5 | 245a | – | ||
Production of heterozygous FBN1 mutant cloned piglets | ||||
Recipient | #R1a | #R2a | #R3b | #R4b |
Embryos transferred | 103 | 103 | 136 | 136 |
Pregnancy | + | + | + | + |
No. of viable cloned piglet | 4 | 3 | 2 | 8 |
No. of stillborn cloned piglet | 0 | 1 | 1 | 0 |
aThe SCNT embryos cultured for 5 to 6 days were transferred to the recipients’ uteri.
bThe SCNT embryos cultured for 1 to 2 days were transferred to the recipients’ oviducts.