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. 2016 Apr 14;6:24378. doi: 10.1038/srep24378

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Copyright © 2016, Macmillan Publishers Limited

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(A) Under non-reducing conditions, rhMMP-9 (lane 1) and purified neutrophil MMP-9/NGAL complex (lane 2) were used as positive controls; lanes 3–5, AVMs; lanes 6–8, normal tissues. (B) Under reducing conditions, purified neutrophil MMP-9/NGAL complex (lane 1) was used as a positive control (MMP-9/NGAL complex will break into MMP-9 and NGAL when running under reducing condition); lanes 2–4, AVMs; lanes 5–7, normal tissues. (C) Under non-reducing conditions, the blot was probed with anti-TIMP-1 antibody, lanes 1–3, AVMs; lanes 4–6, normal tissues. (D) Under reducing conditions, purified neutrophil MMP-9/NGAL complex was loaded as a positive control (lane 1); lanes 2–4, AVMs; lanes 5–7, normal tissues. (E) Under reducing conditions, rhMMP-2 was loaded as a positive control (lane 1); lanes 3–5, AVMs; lanes 6–8, normal tissues. A, AVM; N, normal tissue; M/T, MMP-9/TIMP-1 complex; M/N, MMP-9/NGAL complex.