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. 2016 Apr 14;6:24240. doi: 10.1038/srep24240

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(a) Inhibitor effect on fructose uptake into MCF7 cells. Cytochalasin B (50 or 200 μM) and MSNBA (60 μM) individually or in combination (CytB + MSNBA is 50 μM cytochalasin B and 60 μM MSNBA), were pre-incubated with MCF7 cells for 5 minutes. The uptake assay was initiated by the addition of 10 mM fructose and the transport was stopped after 30 minutes. (b) Dose-dependent MSNBA inhibition of fructose uptake after 30 minutes in MCF7 cells in the presence of 10 mM fructose and 50 μM cytochalasin B. IC₅₀ of MSNBA inhibition was 5.8 ± 0.5 μM. (c) Dixon plot of fructose uptake after 2 minutes into MCF7 cells with varying fructose and MSNBA concentrations, in the presence of 50 μM cytochalasin B. MSNBA displays competitive inhibition of fructose uptake with K_I = 3.2 ± 0.4 μM. Kinetic parameters were determined with Prism (GraphPad Software).