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. 2016 Apr 14;6:24277. doi: 10.1038/srep24277

Figure 3. CES1 is a direct target of HNF4α.

Figure 3

(A) Luciferase reporter assays were performed by co-transfecting HepG2 cells with pGL3-Ces1 luciferase-reporter constructs together with pcDNA3 or pcDNA3-HNF4α plasmids (n = 6). After 36 h, luciferase activities were determined and normalized to β-galactosidase activity. (B) Wild-type (WT) or mutant HNF4α response element in the Ces1 gene promoter is shown in the top or bottom, respectively. (C) EMSA assays were performed using in vitro transcribed/translated HNF4α protein. Wild-type and mutant oligonucleotides were used in the competition assays (left panel). Supershift assays were performed in the presence of an HNF4α antibody (right panel). (D) Chromatin immunoprecipitation assays were performed using liver lysates and an HNF4α antibody. *p < 0.05, **p < 0.01. Unpaired Student t-test was used for statistical analysis.