Skip to main content
. 2016 Apr 14;6:24090. doi: 10.1038/srep24090

Figure 2. HIV-1 Nef interacts with Akt and PI3K.

Figure 2

(A), Nef interacts with Akt and PI3K in PBLs treated with rNef as detected by co-immunoprecipitation. Five million PBLs isolated from healthy donor were left untreated or treated with HIV-1 rNef (100 ng/ml) and subjected to immunoprecipitation with anti-HIV Nef-Ab or isotype control antibody followed by western blot using indicated antibodies (n = 3). (B), Nef interacts with Akt and PI3K via its C-terminus and N-terminus respectively, as measured by pull-down assays. Representative western blot analysis of pull down assay using 1500 μg of PBLs lysates with 20 μg of different HIV-Nef constructs [N-terminus, 1-60aa, C-terminus 55-210aa and full length Nef, 1-210 aa] expressed in BL21DE as indicated. Input corresponds to 10% of the material used for pull down. Presence of PI3K and Akt was detected by respective antibodies mentioned in Material and Method section (n = 3).