Figure 8. rNef induces HIV-1 expression in a latent model cell line 1G5 and protease inhibitor can subsequently block it.

Five million 1G5 cells were either left untreated or treated with rNef in the presence or absence of protease inhibitor. Post 24 h of treatment cells, the expression of luciferase was determined (upper panel) and expression of pAkt was determined by flow cytometry (lower panel). The data shown are representative of two independent experiments.