. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Feb 27;1017-1018:101–113. doi: 10.1016/j.jchromb.2016.02.032

Table 2.

Analyte and internal standard MS/MS optimized parameters on AB Sciex 3200, 4000 QTRAP, and 6500 mass spectrometers.

Analyte	MRM transition (m/z)	Declustering potential	Entrance potential	Collision energy	Exit potential
AB Sciex 3200
bupropion	240.2 > 184.1	31	3.5	15	14
bupropion-d9	249.2 > 185.0	36	5.5	17	14
hydroxybupropion	256.2 > 238.2	36	3.5	17	20
hydroxybupropion-d6	262.2 > 244.2	36	5	17	20
erythrohydrobupropion	242.0 > 168.1	31	4	21	14
erythrohydrobupropion-d9	251.1 > 169.0	41	8	25	3
threohydrobupropion	242.0 > 168.1	31	4	21	14
threohydrobupropion-d9	251.1 > 169.0	41	8	25	3
AB Sciex 4000 QTRAP
bupropion	240.0 > 184.0	56	10	19	28
bupropion-d9	249.3 > 185.1	61	10	19	12
hydroxybupropion	256.1 > 238.1	46	10	17	6
hydroxybupropion-d6	262.3 > 131.1	61	10	43	22
erythrohydrobupropion	242.1 > 168.1	66	10	23	26
erythrohydrobupropion-d9	251.2 > 169.2	106	10	27	28
threohydrobupropion	242.1 > 168.1	66	10	23	26
threohydrobupropion-d9	251.2 > 169.2	106	10	27	28
AB Sciex 6500
bupropion	240.2 > 184.1	31	3.5	15	14
bupropion-d9	249.2 > 185.0	36	5.5	17	14
hydroxybupropion	256.2 > 238.2	36	3.5	17	20
hydroxybupropion-d6	262.2 > 244.2	36	5.0	17	20
erythrohydrobupropion	242.0 > 168.1	31	4.0	21	14
erythrohydrobupropion-d9	251.1 > 169.0	41	8.0	25	3
threohydrobupropion	242.0 > 168.1	31	4.0	21	14
threohydrobupropion-d9	251.1 > 169.0	41	8.0	25	3