Table 3.

Intra-assay and inter- assay accuracy and precision for determination of bupropion and metabolites in human plasma^a on an AB Sciex 3200 mass spectrometer

Analyte	Nominal concentration (ng/mL)	Intra-assay (n=3)			Inter-assay (n=5)
		Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)
(R)-bupropion	2.5	2.5 ± 0.1	101	5	2.3 ± 0.2	92	7
	25	25.0 ± 2.5	100	10	22.3 ± 2.0	91	9
	500	572 ± 58	114	10	481 ± 63	96	13
(S)-bupropion	2.5	2.6 ± 0.2	103	9	2.4 ± 0.2	97	8
	25	24.6 ± 1.8	98	7	22.5 ± 1.1	90	5
	500	577 ± 32	115	5	521 ± 47	104	9
(R,R)-hydroxybupropion	2.5	2.6 ± 0.1	104	4	2.4 ± 0.2	97	7
	25	24.7 ± 1.2	99	5	23.0 ± 0.7	92	3
	500	519 ± 16	104	3	475 ± 30	95	6
(S,S)-hydroxybupropion	2.5	2.2 ± 0.2	89	10	2.3 ± 0.2	90	8
	25	25.3 ± 2.5	101	10	23.1 ± 0.8	92	3
	500	534 ± 16	107	3	481 ± 40	96	8
(1S,2S)-threohydrobupropion	2.5	2.6 ± 0.1	106	5	2.3 ± 0.2	94	11
	25	25.9 ± 3.2	104	12	23.0 ± 0.8	92	4
	500	523 ± 29	105	6	473 ± 38	95	8
(1R,2R)-threohydrobupropion	2.5	2.6 ± 0.1	99	3	2.4 ± 0.2	97	9
	25	24.9 ± 1.9	93	8	22.8 ± 1.3	91	6
	500	538 ± 39	103	7	479 ± 53	96	11
(1R,2S)-erythrohydrobupropion	2.5	2.6 ± 0.2	105	8	2.4 ± 0.3	94	13
	25	25.1 ± 2.1	96	8	22.4 ± 1.4	90	6
	500	544 ± 23	106	4	481 ± 34	96	7
(1S,2R)-erythrohydrobupropion	2.5	2.5 ± 0.3	99	11	2.5 ± 0.4	100	15
	25	24.8 ± 2.7	99	11	23.5 ± 1.0	94	4
	500	531 ± 51	106	10	481 ± 40	96	8

^aCalibration standards were 0.5, 1, 2.5, 5, 10, 25, 50, 100, 250, 500, 1000 ng/mL

^bMean ± standard deviation