Table 4.

Intra- assay and inter- assay accuracy and precision for determination of bupropion and metabolites in human plasma on an AB Sciex 4000 QTRAP mass spectrometer

Analyte	Nominal concentratio n (ng/mL)	Intra-assay (n=3)			Inter-assay (n=5)
		Calculated Concentration (ng/mL)	Accuracy (%)	Precision (%)	Calculated Concentration (ng/mL)	Accuracy (%)	Precision (%)
(R)-bupropion	2.5	2.5 ± 0.3	99	13	2.4 ± 0.2	94	9
	25	23.0 ± 1.1	92	5	23.1 ± 1.5	92	6
	500	517 ± 39	103	8	510 ± 50	102	10
(S)-bupropion	2.5	2.4 ± 0.1	94	2	2.5 ± 0.1	99	3
	25	21.9 ± 0.9	88	4	22.7 ± 1.2	91	5
	500	532 ± 13	106	2	524 ± 44	105	8
(R,R)-hydroxybupropion	2.5	2.3 ± 0.2	94	7	2.4 ± 0.3	96	12
	25	22.0 ± 1.1	88	5	22.8 ± 1.2	91	5
	500	481 ± 22	96	5	481 ± 35	96	7
(S,S)-hydroxybupropion	2.5	2.4 ± 0.3	98	12	2.3 ± 0.3	92	12
	25	23.0 ± 2.5	92	11	22.9 ± 1.3	92	6
	500	490 ± 43	98	9	471 ± 30	94	6
(1S,2S)-threohydrobupropion	2.5	2.4 ± 0.1	94	6	2.4 ± 0.3	96	11
	25	24.3 ± 1.1	97	5	23.1 ± 1.5	93	7
	500	512 ± 27	102	5	472 ± 50	94	11
(1R,2R)-threohydrobupropion	2.5	2.3 ± 0.2	90	8	2.4 ± 0.3	97	14
	25	23.7 ± 2.5	95	11	23.0 ± 1.0	92	4
	500	519 ± 38	104	7	481 ± 42	96	9
(1R,2S)-erythrohydrobupropion	2.5	2.6 ± 0.3	105	10	2.3 ± 0.3	90	12
	25	23.6 ± 2.1	94	9	21.6 ± 1.3	87	6
	500	480 ± 18	96	4	461 ± 56	92	12
(1S,2R)-erythrohydrobupropion	2.5	2.3 ± 0.2	94	7	2.4 ± 0.3	95	11
	25	22.9 ± 2.6	91	11	23.3 ± 1.7	93	7
	500	490 ± 39	98	8	487 ± 34	97	7

Results are the mean ± standard deviation