Table 5.

Intra-assay and inter-assay accuracy and precision for determination of bupropion and metabolites in human plasma on an AB Sciex 6500 mass spectrometer

Analyte	Nominal concentratio n (ng/mL)	Intra-assay (n=3)			Inter-assay (n=5)
		Calculated Concentration (ng/mL)	Accuracy(%)	Precision (%)	Calculated Concentration (ng/mL)	Accuracy (%)	Precision (%)
(R)-bupropion	2.5	2.2 ± 0.1	88	5	2.3 ± 0.3	93	11
	25	21.1 ± 1.1	85	5	22.5 ± 1.5	90	7
	500	494 ± 13	99	3	498 ± 35	100	7
(S)-bupropion	2.5	2.3 ± 0.2	91	7	2.4 ± 0.3	96	11
	25	21.6 ± 1.4	86	7	22.7 ± 1.7	91	8
	500	522 ± 11	104	2	534 ± 52	107	10
(R,R)-hydroxybupropion	2.5	2.1 ± 0.1	84	7	2.3 ± 0.3	91	14
	25	20.6 ± 1.3	82	6	21.9 ± 1.8	88	8
	500	518 ± 23	104	4	534 ± 34	107	6
(S,S)-hydroxybupropion	2.5	2.3 ± 0.0	93	2	2.3 ± 0.3	93	11
	25	20.9 ± 1.7	84	8	21.7 ± 2.0	87	9
	500	485 ± 7	97	1	480 ± 28	96	6
(1S,2S)-threohydrobupropion	2.5	2.4 ± 0.1	94	6	2.4 ± 0.2	96	10
	25	22.2 ± 1.1	89	5	23.0 ± 1.2	92	5
	500	457 ± 43	91	9	485 ± 39	97	8
(1R,2R)-threohydrobupropion	2.5	2.3 ± 0.2	90	7	2.4 ± 0.2	96	9
	25	21.7 ± 0.7	87	3	22.8 ± 1.2	91	5
	500	492 ± 9	98	2	493 ± 36	99	7
(1R,2S)-erythrohydrobupropion	2.5	2.3 ± 0.1	91	6	2.3 ± 0.3	91	14
	25	22.6 ± 0.6	90	3	22.9 ± 1.6	92	7
	500	478 ± 15	95	3	485 ± 49	97	10
(1S,2R)-erythrohydrobupropion	2.5	2.3 ± 0.2	91	7	2.4 ± 0.3	96	12
	25	21.6 ± 1.2	87	6	22.7 ± 1.5	91	7
	500	483 ± 6	97	1	489 ± 42	98	9

Results are the mean ± standard deviation