Table 6.

Intra-day and inter-day accuracy and precision for determination of bupropion and metabolites in human plasma with analyte specific calibration ranges^a on an AB Sciex 3200 mass spectrometer

Analyte	Nominal concentration (ng/mL)	Intra-assay (n=5)			Inter-assay (n=5)
		Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)	Calculated Concentration (ng/mL)b	Accuracy (%)	Precision (%)
(R)-bupropion	2.5	2.5 ± 0.2	99	6	2.6 ± 0.3	106	10
	10	9.8 ± 0.3	98	3	9.9 ± 0.5	99	5
	25	25.5 ± 1.0	102	4	25.8 ± 1.2	103	5
	50	49.1 ± 2.3	98	5	49.6 ± 3.1	99	6
(S)-bupropion	2.5	2.1 ± 0.1	97	6	2.5 ± 0.1	100	3
	10	7.8 ± 0.5	94	6	9.4 ± 0.4	94	5
	25	20.8 ± 0.9	100	4	25.7 ± 0.9	103	3
	50	41.1 ± 1.7	100	4	49.2 ± 1.9	98	4
(R,R)-hydroxybupropion	5	4.8 ± 0.4	96	9	5.1 ± 0.2	102	3
	25	27.7 ± 2.0	111	7	27.6 ± 1.1	110	4
	250	260 ± 9	104	4	262 ± 12	105	5
	1000	969 ± 38	97	4	964 ± 42	96	4
	2000	1990 ± 151	100	8	2035 ± 121	102	6
(S,S)-hydroxybupropion	5	5.0 ± 0.3	100	7	5.1 ± 0.3	102	5
	25	26.1 ± 1.2	104	5	26.1 ± 0.1	105	0
	250	248 ± 10	99	4	246 ± 5	98	2
	1000	963 ± 33	96	3	955 ± 29	96	3
(1S,2S)-threohydrobupropion	2.5	2.5 ± 0.2	100	9	2.5 ± 0.1	100	5
	10	9.7 ± 0.6	97	6	9.8 ± 0.2	98	2
	50	49.0 ± 2.1	98	4	50.0 ± 2.1	100	4
	200	200 ± 6	100	3	201 ± 3	100	1
(1R,2R)-threohydrobupropion	2.5	2.5 ± 0.2	99	10	2.5 ± 0.0	99	2
	10	9.6 ± 0.5	96	5	9.8 ± 0.5	98	5
	50	49.2 ± 2.4	98	5	50.8 ± 1.8	102	3
	200	200 ± 7	100	4	202 ± 8	101	4
(1R,2S)-erythrohydrobupropion	2.5	2.5 ± 0.3	100	12	2.6 ± 0.2	105	8
	10	9.6 ± 0.7	96	7	9.8 ± 0.3	98	3
	50	48.2 ± 2.5	96	5	50.8 ± 2.0	102	4
	200	197 ± 8	99	4	198 ± 6	99	3
(1S,2R)-erythrohydrobupropion	2.5	2.5 ± 0.2	100	7	2.5 ± 0.1	100	4
	10	9.9 ± 0.5	99	5	9.9 ± 0.3	99	3
	50	48.6 ± 2.0	97	4	50.6 ± 1.7	101	3
	200	201 ± 6	101	3	203 ± 4	101	2

^aCalibration ranges are provided in Table 1

^bMean ± standard deviation