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Preparation of samples for mass spectrometric analysis. Following the expansion of ASCs from three donors for 72 h, cells were cultured under either normoxic or hypoxic conditions for 24 h. The conditioned media were harvested and sequentially fractionated through 30-kDa and 3-kDa spin filters to retain the secretome and peptidome fractions, respectively. The cellular fraction was employed for the analysis of the proteome. ASC adipose-derived stem cell
