Fig. 2.

Effects of culture supplements on the growth and viability of the ASCs. a Growth curves of ASCs expanded for 3 days in the different media formulations. At day 3, there was a significantly higher number of cells in the StemPro + cultures as compared to the cultures with the other two media formulations (***p < 0.001, n = 6). b Cells expanded for 3 days in StemPro + and preconditioned for 24 h in the different media. After preconditioning, the number of cells in StemPro– cultures had significantly decreased in relation to the StemPro + cultures (**p < 0.01, n = 6). The percentage of viable cells was equivalent among the different formulations. c Phase contrast microphotographs displaying the morphology of cells after the preconditioning period in different media. The figure shows representative pictures from one donor. Scale bar = 200 μm